Maternal RNF114-mediated target substrate degradation regulates zygotic genome activation in mouse embryos

ABSTRACT Zygotic genomic activation (ZGA) is a landmark event in the maternal-to-zygotic transition (MZT), and regulation of ZGA by maternal factors remains to be elucidated. In this study, depletion ring finger protein 114 (RNF114), ubiquitin E3 ligase, led developmental arrest two-cell mouse embryos. Using immunofluorescence transcriptome analysis, RNF114 was proven play crucial role major ZGA. To study underlying mechanism, we performed profiling mature oocytes found potential substrate for RNF114, chromobox 5 (CBX5), ubiquitylation degradation which regulated RNF114. The overexpression CBX5 prevented embryonic development impeded Furthermore, TAB1 abnormally accumulated mutant embryos, consistent with result vitro knockdown Rnf114. Knockdown Cbx5 or Tab1 RNF114-depleted embryos partially rescued defect summary, our reveals that plays precise degrading some important substrates during MZT, misregulation may impede appropriate